Skip to main content
. 2017 Jan 3;3:16048. doi: 10.1038/celldisc.2016.48

Figure 1.

Figure 1

TDRD3 interacts with USP9X. (a) GST pull-down assays were performed using recombinant GST, GST-Tudor and GST-Tudor (E691K) proteins with the HeLa cell total cell lysates. Both the input samples and pull-down samples were detected with an anti-USP9X antibody (left panel). The GST-tagged recombinant proteins in the pull-down samples were visualized by Ponceau S staining (right panel). (b) TDRD3 and USP9X co-IP. HeLa cells were IPed with rabbit control IgG and two different rabbit polyclonal anti-TDRD3 antibodies. Both the input and the eluted protein samples were detected with anti-TDRD3 and anti-USP9X antibodies. Two different sources of TDRD3 antibody were used to confirm the results—anti-TDRD3 serum [13] and TDRD3 antibody from Cell Signaling Technology (Danvers, MA, USA) (TDRD3 CST). (c) TOP3B does not interact with USP9X. Both the HeLa cells and HEK293 cells were IPed with rabbit control IgG, anti-TDRD3 and anti-TOP3B antibodies and detected with anti-TDRD3 and anti-USP9X antibodies. (d) HeLa cells transiently transfected with GFP empty vector, GFP-TDRD3 and GFP-TOP3B were IPed with an anti-GFP antibody. The input and IPed protein complexes were detected with anti-GFP and anti-USP9X antibodies.