Figure 5.

TDRD3 regulates USP9X localization to SGs in response to arsenite treatment. (a) HeLa cells cultured on glass coverslips were left untreated (Control) or treated with 0.5 mm sodium arsenite (Arsenite) for 30 min. The cells were fixed and immunostained with anti-USP9X and anti-TDRD3 to detect the localization of endogenous proteins. DAPI was used to stain the nuclear DNA. The arrows indicate USP9X and TDRD3 co-localization in SGs. (b) HeLa cells were treated as in a and immunostained with anti-USP9X and anti-G3BP. (c) Wild-type and TDRD3-knockout MEF cells were both treated with 0.5 mm sodium arsenite for 30 min. The cells were fixed and immunostained with anti-USP9X and anti-G3BP antibodies.