Figure 3. SHL reduced Ca²⁺_[c] levels.

(A) Effect of SHL on IgE-mediated Ca²⁺_[c] level in the sensitized RBL-2H3 cells. The sensitized cells were loaded with fluo-3 AM (4 μM) at 30 °C for 30 min. The stained cells were treated with or without SHL for 30 min and then exposed to ST (20 ng/ml). The fluorescent intensity (λ_ex 485 nm and λ_em 538 nm) was recorded every 30 s. ^##P < 0.01 vs. control; ^*P < 0.05, ^**P < 0.01 vs. ST alone. (B) SHL reduced Ca²⁺_[c] levels in the resting RBL-2H3 cells. The cells were loaded with fluo-3 AM (4 μM) at 30 °C for 30 min. The stained cells were treated with or without SHL and the fluorescent intensity (λ_ex 485 nm and λ_em 538 nm) was immediately recorded. ^**P < 0.01 vs. control.