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. 2017 Jan 3;7:39335. doi: 10.1038/srep39335

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(a and c) Immunostaining of activated (phosphorylated) Smad2 (a) and TAK1 (c) in the control and afadin cKO hearts during pressure overload challenge. Images below the respective phospho-TAK1 photos showing magnification of depicted rectangular areas. Wheat germ agglutinin (WGA: red) staining for the cell membranes, and DAPI (blue) staining for the nuclei. Arrowheads: disturbed myocardial structure. (b and d) Summary graphs for percentage of phospho-Smad2-positive nuclear area (b) and phopsho-TAK1-positive cytosolic area (d), which were estimated per 1000 nuclei and cells in each group. *p < 0.05.