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. 2017 Jan 3;7:39335. doi: 10.1038/srep39335

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Copyright © 2017, The Author(s)

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(a and b) Immunoprecipitation using indicated antibodies to examine the association of afadin and TGFβ receptor I (TGFβRI) in the samples from the control mouse hearts (a) and from COS7 cells transfected with GFP-afadin (b). IP: immunoprecipitation, IB: immunoblot for immunoprecipitated samples. Asterisks: IgG heavy chain. The images are representative from at least 3 separate experiments. (c) Co-immunostaining of afadin (green) and TGFβRI (red). Image on the right side: the enlargement of area surrounded by dotted rectangle. DAPI (blue) staining for the nuclei. (d) Mean values of Pearson correlation coefficient and Manders split coefficients obtained from confocal images (c) in order to demonstrate positive co-localization between afadin and TGFβ receptor I in control hearts. (e) Immunoprecipitation experiments to evaluate the time course of afadin-TGFβRI association during chronic pressure overload. (f) Summary graph of the afadin-TGFβRI association ratio, which were normalized to the sample before (0 week) TAC (n = 5 in each group). *p < 0.05.