Figure 2.
Role of PMN in HIF stabilization during mucosal inflammation. Tissue from the distal colons of healthy mice and mice with active TNBS colitis, with and without depletion of systemic PMN through the use of anti-GR-1 antibody, reveals an increase in HIF among colitic mice with PMN, which is attenuated in the absence of PMN. Staining for HIF ( green) and Ly6g (red ) indicates accumulation of PMN in TNBS colitis. DAPI stains (blue) signify areas of high DNA concentration. Scale bars = 100 μm. Abbreviations: HIF, hypoxia-inducible factor; PMN, polymorphonuclear leukocytes; TNBS, trinitrobenzene sulfonic.