Fig. 2.

Tumorigenesis caused by Raf^gof can be effectively inhibited by induction of autophagy in the Drosophila adult midgut. (A) Wildtype progenitors expressing esgGal4, UAS-GFP (green) in midguts at 29 °C for 3 days (white arrowheads). (B) No obvious defects are observed in esg^ts>Atg1 intestines (white arrowheads). (C) Intestinal tumors in esg^ts>Raf^gof intestines at 29 °C for 3 days (white arrowheads). (D) Tumor formation in esg^ts>Raf^gof intestines is significantly inhibited by co-expressing Atg1 (white arrowheads). (E) Quantification of the number of esg+ cells in the intestines of different genotypes. Note that because esg^ts>Raf^gof intestines are highly deformed due to the formation of tumors, it is very difficult to accurately count the number of esg+ cells in these intestines. n=10–15 intestines. Mean ± SD is shown. **p<0.001. (F) Quantification of pH3 staining per gut in the intestines of different genotypes. n=10–15 intestines. Mean ± SD is shown. **p<0.001. Blue indicates DAPI staining for DNA. Scale bars: 20 μm.