Fig. 3.

Tumor formation in the absence of Notch signaling can be effectively inhibited by induction of autophagy in the adult Drosophila midgut. (A) ISC MARCM clones (green) in FRT control 6 days after clone induction (6D ACI) (white arrowheads). (B) Progenitor tumors (green) are formed in N^264-39 ISC clones (6D ACI) (white arrowheads). (C). ISC MARCM clones (green) expressing Atg1 (6D ACI) (white arrowheads). (D) Progenitor tumors observed in N^264-39 ISC clones are significantly inhibited by co-expression of Atg1 (white arrowheads). (E) Progenitor tumors (green) are formed in Dl^RevF10 ISC clones (6D ACI) (white arrowhead). (F) Progenitor tumors observed in Dl^RevF10 ISC clones are almost completely inhibited by co-expression of Atg1 (white arrowheads). (G) Quantification of relative size of ISC clones (cell/clone) in the intestines of different genotypes. Note that both N^264-39 and Dl^RevF10 ISC clones are highly deformed, making it difficult to accurately count the number of mutant cells. n=5–10 intestines. Mean ± SD is shown. **p<0.001. Blue indicates DAPI staining for DNA. Scale bars: 20 μm.