Fig. 4.

Atg1-mediated suppression of Raf^gof tumor formation does not rely on caspase-dependent apoptosis. (A) Progenitors in esg^ts>p35 midgut at 29 °C for 3 days (white arrowheads). (B) No obvious effects are observed in intestines co-expressing Atg1 and p35 at 29 °C for 3 days (white arrowheads). (C) Intestinal tumors are formed in esg^ts>Raf^gof intestines at 29 °C for 3 days (white arrowheads). (D) ISCs in esg^ts>p35; Raf^gof midgut at 29 °C for 3 days (white arrowheads). (E) Atg1-mediated suppression of Raf^gof tumor formation cannot be rescued by co-expression of p35 (white arrowheads). (F) Quantification of the number of esg+ cells in the intestines of different genotypes. Note that because esg^ts>Raf^gof intestines are highly deformed due to the formation of tumors, it is very difficult to accurately count the number of esg+ cells in these intestines. n=10–15 intestines. Mean ± SD is shown. **p<0.001. (G) Quantification of pH3 staining per gut in the intestines of different genotypes. n=10–15 intestines. Mean ± SD is shown. **p<0.001. Blue indicates DAPI staining for DNA. Scale bars: 20 μm.