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. Author manuscript; available in PMC: 2017 Aug 5.
Published in final edited form as: Vaccine. 2016 Aug 5;34(36):4285–4292. doi: 10.1016/j.vaccine.2016.07.010

Fig. 4.

Fig. 4

Serum immune responses and protection efficacy in orally immunized mice. Total serum IgG specific for S. Typhimurium LPS (A) and serum IgG against OMPs from S. Typhimurium and other enteric bacteria (B) were measured via quantitative ELISA. Each group consisted of 12 mice. The data represent antibody concentrations in pooled sera from orally immunized mice at the indicated number of weeks post-immunization (***P < 0.001, S492 (ΔrfbB6 ΔrffG8) versus S496 (PBAD rfbB-3)). The protection rates against S. Choleraesuis (C) (n = 6/group) and S. Enteritidis (D) (n = 6/group) challenge are shown. The immunized mice were challenged by oral inoculation with 200 × LD50 S. Choleraesuis and 200 × LD50 S. Enteritidis. The mice were monitored daily and deaths were recorded for 30 days to calculate the protection rates after challenge. Statistical analyses were performed using the log-rank test.