Table 1.
Strains and plasmids |
Description | Source or reference |
---|---|---|
Plasmids | ||
pYA4278 | SacB mobRP4 R6K ori Cm+, pRE112-T-vector | [6] |
pSS239 | ΔrfbB6 | pYA4278 |
pSS240 | ΔrffG8 | pYA4278 |
pYA4284 | ΔpagL7 | [35] |
pSS242 | ctcgag AGGA gtcatt GTG | pSS241 |
pSS243 | ctcgag AGGA gtcatt ATG | pSS241 |
pSS244 | ctcgag GGAA gtcatt ATG | pSS241 |
pSS245 | ctcgag GGAA gtcatt GTG | pSS241 |
pYA4518 | GFP p15A ori Cm+ | [37] |
pYA3700 | TT araC PBAD cassette plasmid; Apr | [39] |
pSS250 | pYA4518-rfbB | |
pSS251 | pYA4518-rffG | |
Bacterial strains | ||
S100 | Wild-type S. Typhimurium, isolated from infected duck |
[40] |
S340 | S. Choleraesuis, wild-type virulent | [40] |
S246 | S. Enteritidis, wild-type virulent | [40] |
S490 | ΔrfbB6 | S100 |
S491 | ΔrffG8 | S100 |
S492 | ΔrfbB6 ΔrffG8 | S491 |
S493 | ΔrfbB6 ΔrffG7 ΔpagL7 | S492 |
S494 | ΔrfbB6 ΔrffG7 ΔpagL71:TT araC PBAD rfbB-1 | S493 |
S495 | ΔrfbB6 ΔrffG7 ΔpagL72:TT araC PBAD rfbB-2 | S493 |
S496 | ΔrfbB6 ΔrffG7 ΔpagL73:TT araC PBAD rfbB-3 | S493 |
S497 | ΔrfbB6 ΔrffG7 ΔpagL74:TT araC PBAD rfbB-4 | S493 |
E. coli | ||
χ7232 |
endA1 hsdR17 () glnV44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 λpir deoR (Φ80dlac Δ (lacZ)M15) |
[41] |
χ7213 |
thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4- 2-Tc::Mu[λ pir] ΔasdA4 Δ(zhf-2:Tn10) |
[41] |