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. 2017 Jan 10;26(2):49–69. doi: 10.1089/ars.2016.6769

FIG. 5.

FIG. 5.

Effect of Del treatment on TPA-induced NHEK secretion of proinflammatory cytokines and human neutrophil chemotactic activity in vitro. (A) NHEKs were cotreated with/without Del (10–20 μM) and/or TPA and cytokine production in supernatants was analyzed by Procarta 6-plex mix-matched immunoassay. Del (10 and 20 μM) dose-dependently inhibited TPA-induced NHEK secretion of the indicated cytokines. (B) Chemotactic activity for circulating human neutrophils using supernatants from TPA-stimulated NHEKs treated or not with Del (10 and 20 μM). Percentage inhibition of neutrophil migration using supernatant from Del-treated cells (10 and 20 μM) compared with TPA only is shown. (A, B) Data are mean ± SEM. *denotes p < 0.05; **denotes p < 0.01; and ***denotes p < 0.001, ****denotes p < 0.0001. For neutrophil chemotaxis in (B), graph is for three different keratinocyte-conditioned media and three different neutrophil donors. * indicates that migration is significantly inhibited by Del. For (A), results are representative of three independent experiments.