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. 2017 Jan 3;7:218. doi: 10.3389/fgene.2016.00218

FIGURE 1.

FIGURE 1

(A) R8 congenic animals possess a small 1.1 Mb donor (D2, gray) segment from distal chromosome 1 on an inbred B6 (white) genetic background. The reciprocal congenic, R2, possesses a larger 10.2 Mb donor (B6, white) interval from the same region of distal chromosome 1 on an inbred D2 (gray) genetic background. Boundary regions (within which the recombination site is located) are indicated in black. (B) Mice were scored twice for baseline HICs (indicated by B) immediately before administration of 4 g/kg ethanol (arrow = injection at time 0). Post-ethanol HICs were scored hourly from 2 to 2 h post-ethanol in R2 homozygotes (black squares), R2 heterozygotes (gray squares), and WT littermates (open squares) (N = 27, 44, and 16 mice per genotype, respectively). After 4–5 h, convulsion scores increased above baseline, indicating a state of withdrawal hyperexcitability that peaks about 6–8 h post-administration. Data represent the HIC score (mean ± SEM). (C) Alcohol withdrawal severity scores (corrected mean AUC ± SEM) for R2 homozygote, R2 heterozygote and WT littermates are shown. ∗∗Significantly different (p < 0.005) than WT littermates; #Significantly different (p < 0.01) than R2 homozygotes.