Table 1.

Primers used in this study.

Name	Sequence (5′–3′)	Amplicon length	Gene (mutation*)	References
BpsID_137F	GCAGATGAGGCGGATGATAG	137/112 bp**	BC1G_07159/Bcin09g02270	Plesken et al., 2015a
BpsID_273R	TCCACCCAAGCATCATCTTC
BcinN-in-F	GCGACCTCATCGTTCTTTCAC	182 bp	mrr1	Plesken et al., 2015a
BcinN-in-R	GGCTCTCGATGAGCTGTTTC
Mrr1-spez-F	TATCGGTCTTGCAGTCCGC	141 bp	mrr1	Leroch et al., 2013
Mrr1-spez-R	TTCCGTACCCCGATCTTCGGAA
erg27F412SP745F	GTGTTGGTATTTATCTACAGATTGATAT	125 bp	erg27 (F412S*1)	This work
erg27_1410R	ACATCGTCGGGAGATAATGC
Seq_erg27_fw	GTACCGCCACTTATTCCG	950 bp	erg27 (F412I)	This work
Seq_erg27_rev	GCGTTCTTTCCCACTAGC
Seq_erg27_I63	CAGGCGCAAACAGGTGATAC	1425 bp	erg27 (T63I)	This work
Seq_erg27_rev	GCGTTCTTTCCCACTAGC
Qo13ext	GGTATAACCCGACGGGGTTATAGAATAG	560 bp***	cytB (G143A*2)	Samuel et al., 2011
Qo14ext	AACCATCTCCATCCACCATACCTACAAA
H272R-fw	GGCAGCTTTGGATAACAGCATGAGTTTGTACAGATGGC	120 bp	sdhB (H272R*3)	Veloukas et al., 2011
H272-univ-rev	GCCATTTCCTTCTTAATCTCCGC
H272Y-fwfw	GGCAGCTTTGGATAACAGCATGAGTTTGTACAGATAT	120 bp	sdhB (H272Y*4)	Veloukas et al., 2011
H272-univ-rev	GCCATTTCCTTCTTAATCTCCGC
Mrr1_spez_F	TATCGGTCTTGCAGTCCGC	442 bp	mrr1 (ΔL497*5)	Leroch et al., 2013
Mrr1-Pira	CCACCACAATCTTGGATCATTGGGATCAGAACCTGC
PIRA-Tub1	GCCTCGTTATCGATACAGAAGATC	132 bp	tubA (E198A*6)	Plesken et al., 2015a
PIRA-Tub2	TATGATGGCTACCTTCTCCGTC
BF2	CAACGTTATGGCACAAAATCTCA	849 bp	bos1	Ma et al., 2007
BR2	AAGTTTCTGGCCATGGTGTTCA

^*Fungicide resistance mutation detected.

^**Amplicon sizes for B. cinerea and B. pseudocinerea, respectively.

^***Amplicon size if no intron is present.

The following enzymes were used for digestion of the PCR fragments to detect the indicated mutation:

^*1EcoRV;

^*2SatI;

^*3HhaI;

^*4EcoRV;

^*5HpyCH4V;

^*6BglII.