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. Author manuscript; available in PMC: 2018 Jan 2.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Dec 2;56(1):212–216. doi: 10.1002/anie.201609452

Figure 4. Probing functions of epigenetic enzymes with synthetic dimethyl-H3 and p53 proteins.

Figure 4

(A) LSD1 activities on three dimethyl-H3 variants. 1 µg H3K4me2, H3K9me2, and H3K36me2 were incubated with 0.1 µg for 8 h before they were analyzed in SDS-PAGE and probed by anti-Kme2 and anti-H3 antibodies in western blotting. (B) Methylation at p53 K372 activates Tip60-catalyzed acetylation at p53 K120. 0.1 µg wild type (wt) p53, p53-K372me, and p53-K372me2 was incubated with 0.01 µg Tip60 for 2 h before they were analyzed by SDS-PAGE and probed with antibodies such as anti-p53-K372me, anti-Kme2, and anti-p53-K120ac in western blotting. (C) Quantified activation effects of p53 K372 methylation on p53 K120 acetylation by Tip60.