Figure 3.

KDM5B is a direct target of miR-194. (a) Bioinformatics-based target prediction analysis shows that KDM5B is a potential target of miR-194. (b) Luciferase reporter assay shows that in the miR-194 mimic group, the luciferase activity driven by 3′UTR of KDM5B is obviously decreased compared with that in the negative control and 3′UTR-MUT group. (c) qRT-PCR shows that miR-194 level is lower in HEEpiC transfected with miR-194 inhibitors than in control cells. (d) Down-regulation of miR-194 in HEEpiC cells increased KDM5B protein expression, and up-regulation of miR-194 in TE6 cells significantly decreased KDM5B protein expression. (e) CCK-8 assay shows that the siKDM5B group has a lower cell proliferation rate than si-control group. (f) Flow cytometer assay shows that the siKDM5B group has a significantly higher apoptosis rate than si-control group. (g) Transwell chamber shows that the siKDM5B group has a markedly weaker invasion capacity than si-control group. (h) Western blot assay shows that the siKDM5B group has obvious decreases in Ki67, Bcl-2, MMP-2 and MMP-9 protein expression, and significant increase in Bax protein expression compared with si-control group. *P < 0.05, **P < 0.01 and ***P < 0.001