Skip to main content
. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Leuk Lymphoma. 2016 Jun 7;58(2):445–456. doi: 10.1080/10428194.2016.1190973

Figure 4. ZF-KRAB proteins cause repressive chromatin changes at the BCL6 locus.

Figure 4

A) Schematic of the BCL6 locus, including ChIP-qPCR regions (labeled 1 – 4) and binding sites for ZFe1- (exon 1, #2) and ZFp-KRAB (promoter, #3) proteins. B) H3ac ChIP assays in OCI-Ly7 cells cultured for three days with or without doxycycline to induce expression of the ZFe1- or ZFp-KRAB fusion protein. Associated DNA was analyzed via qPCR using primers spanning the following regions: ZFe1-KRAB binding site in exon 1 of BCL6 (2), ZFp-KRAB binding site in the promoter of BCL6 (3), sites 3kb downstream (1) or 1.5kb upstream (4) of the BCL6 TSS, and a control site at the TUBA1B gene. H3ac was normalized to total histone H3. Representative of three independent experiments. C) H3K4me3 ChIP assays in OCI-Ly7 cells cultured and treated as in B. Associated DNA was analyzed via qPCR and normalized to total histone H3 as in B. Representative of three independent experiments.