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. Author manuscript; available in PMC: 2017 Jan 3.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Jul 25;478(2):559–564. doi: 10.1016/j.bbrc.2016.07.100

Fig. 4. Kinetics (Km and Vmax) of rat cardiac chymase for Ang-(1-12) and Ang I substrates.

Fig. 4

PMs (100 µg) were incubated with increasing concentrations (5–300 µM) of Ang-(1-12) or Ang I substrate in the presence of lisinopril (200 µM, ACE inhibitor) at 37° C for 30 min. The Ang II generated was analyzed by HPLC connected to UV-detector at 215 nm. The Km and Vmax of cardiac chymase for Ang-(1-12) and Ang I substrates were calculated using the Michaelis-Menten equation.