Figure 1.
miR-22 and -378 are downregulated in RMS but are induced during myogenic differentiation. A, section of the expression dendrogram including the top 8 differentially expressed miRNAs in human skeletal muscles compared to RMS cells and tissues. Yellow indicates increased expression; black indicates reduced expression. B, Northern blot analysis on total RNA obtained from one muscle and one RMS sample. C and D, real-time PCR analysis in murine satellite cells (C) and C2C12 cells (D) grown in proliferation ‘P’ or differentiation medium ‘D’. E and F, real-time PCR analysis in inducible miR-206-expressing RD18 (NpBI-206) cells (E) and MyoD-expressing NIH 10T½ (NpBI-MyoD) cells (F) (induced, IND; non-induced, NI). Error bars, SD.