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. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: Cancer Res. 2016 Aug 28;76(20):6095–6106. doi: 10.1158/0008-5472.CAN-16-0709

Figure 3.

Figure 3

miR-22 is transcriptionally regulated by MyoD. A, schematic representation of miR-22 promoter region. The putative binding sites for MyoD are indicated by black boxes. B and C, ChIP analysis of miR-22 promoter regions in NIH NpBI-MyoD cells (induced, IND; non-induced, NI). Two independent oligo pairs were used for miR-22 promoter amplification (oligos A and oligos B). MCK and IgH enhancers were used as positive and negative controls respectively. Recruitment was relative to normal rabbit IgG. D, dual luciferase assay in NIH NpBI-MyoD cells (induced, IND; non-induced, NI) transfected with the indicated luciferase reporter constructs. Error bars, SD. *P<0.05; **P<0.01; ***P<0.001 (t test).