Figure 6.
RAB5B controls RMS cell migration. A and B, real-time PCR analysis of RAB5 paralog transcripts in RD18 (A) and RH30 (B) cells. C, Western blot analysis in RD18 cells transfected with siRNA control (siCTRL) or with RAB5-directed siRNAs (siRAB5A, B or C). D and E, representative images (D) and quantification (E) of wound closure of RD18 cells transfected with siCTRL or siRAB5B. F, Western blot analysis in RD18 NpBI-22 cells expressing GFP (CTRL) or RAB5B. G and H, representative images (G) and quantification (H) of wound closure of RD18 NpBI-22 cells maintained in presence of doxycycline (induced, IND) and expressing GFP (CTRL) or RAB5B. Error bars, SEM. *P<0.05 (t test).