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. Author manuscript; available in PMC: 2017 Jan 3.
Published in final edited form as: Methods Mol Biol. 2014;1108:3–21. doi: 10.1007/978-1-62703-751-8_1

Table 2.

Na2HPO4–NaH2PO4 buffer solutions, pH 6.8–8.0 at 25 °C [21]

pH, 25 °C xml 0.2 M Na2HPO4 yml 0.2 M NaH2PO4
7.0 30.5 19.5
7.2 36.0 14.0
7.4 40.5 9.5
7.6 43.5 6.5
7.8 45.75 4.25
8.0 47.35 2.65

xml 0.2 M Na2HPO4, yml 0.2 M NaH2PO4; diluted to 100 ml with H2O → 0.1 M solution

x/2 ml 0.2 M Na2HPO4, y/2 ml 0.2 M NaH2PO4; diluted to 100 ml with H2O → 0.05 M solution

xml 0.2 M Na2HPO4, yml 0.2 M NaH2PO4; diluted to 1,000 ml with H2O → 0.01 M solution