FIGURE 4.

Aer oligomerization affects activity. A, higher molar ratios of Aer to MSP1E3D1 increases Aer loading into nanodiscs. The analytical size exclusion chromatograms for two preparations (Prep) of Aer into nanodiscs are shown after the areas of the peaks have been normalized. Larger particles with a higher average number of Aer dimers per nanodisc result from increased Aer:MSP1E3D1 ratios. The solid black line marks the position of the void peak. Abs., absorbance; AU, arbitrary units. B, CheA activities in the presence of various Aer preparations are compared. The activities are measured relative to the control sample (designated by 0), which contains only CheA, CheW, and CheY. The continuous line is drawn for the aid of inspection. Individual data points are shown as open black circles. The error bars are S.E. with n ≥ 4.