FIGURE 2.

mTORC1 regulation in T cells in low amino acid environments. A and B, time course of pS6, S6, or p-mTOR in gated CD4⁺ cells from an MLR assay. The dotted line indicates the time zone of the first division defined by the independently performed CFSE dye dilution assay (B). C, time course of p-4E-BP1 in gated CD4⁺ T cells as in A where cultures were in normal RPMI or RPMI with 1% arginine or 1% leucine. D, time course of pS6 in gated CD4⁺ T cells as in C. E, quantification of flow data from the experiment in panels C and D. Experiments were done 3 independent times for arginine starvation. Panel B is the internal CFSE control experiment for data in panel A. Data in panel E is a summary figure from a single representative experiment.