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. 2016 Oct 26;291(50):25823–25836. doi: 10.1074/jbc.M116.756908

FIGURE 8.

FIGURE 8.

The effect of HuR and AUF1 on ATX expression in B16 melanoma cells. A and B, the effects of HuR knockdown and HuR overexpression on ATX expression in B16 cells. B16 cells were transfected with HuR siRNA (A) or the plasmid expressing FLAG-HuR (B). At 48 h after the transfection, ATX protein and mRNA expression levels were assessed by Western blotting and RT-qPCR, respectively. C and D, the effects of AUF1 knockdown or AUF1 overexpression on ATX expression in B16 cells. B16 cells were transfected with AUF1 siRNA (C) or the plasmid pcDNA3.1-AUF1 (D). At 48 h after the transfection, ATX protein and mRNA expression levels were assessed by Western blotting and RT-qPCR, respectively. E, the functional interaction between HuR and AUF1 in ATX expression regulation. B16 cells were transfected with HuR siRNA and/or AUF1 siRNA as indicated. At 48 h after the transfection, ATX protein and mRNA levels were assessed by Western blotting and RT-qPCR. *, p < 0.05, **, p < 0.01.