FIGURE 9.

The effects of HuR/AUF1-mediated ATX post-transcriptional regulation on B16 cell migration. A, scratch wound-healing assay. B16 cells treated with the indicated siRNA and/or the chemical compound were used for the scratch wound-healing assay. 1, NC siRNA; 2, NC siRNA and ATX inhibitor S32826 (2 μm); 3, HuR siRNA; 4, HuR siRNA and LPA (2 μm); 5, HuR siRNA and AUF1 siRNA. Images of the cells migrating into the wound were captured at 0 and 12 h by an inverted microscope (×10). The relative migration rate was calculated by dividing the change in the gap distance between the scratch edges by the initial distance. B, transwell assay. B16 cells were treated with the indicated siRNA and/or the chemical compound as described in A and subjected to the transwell assay. Images of cells on the lower surface of the upper chamber were taken 24 h after the cells were seeded into the upper chamber. The relative migration was calculated by counting the cell number in the lower surface of the upper chamber, and data were obtained from three randomly chosen fields. Representative data were from three independent experiments. The p value derived from Student's t test is *, p < 0.05 and **, p < 0.001.