FIGURE 6.

ATPase activity of engineered Spa47 constructs. Kinetic analysis of ATP hydrolysis by each of the engineered Spa47 constructs used in this study. Wild-type Spa47 monomeric and oligomeric species are ATPase active with an increase in hydrolysis efficiency for the Spa47 oligomer. Isolated monomeric and oligomeric forms of Spa47^K165A, Spa47^E188A, and Spa47^R350A, as well as the monomeric form of the oligomerization-deficient Spa47^Δ1–79 construct were all inactive, resulting in background levels of ATP hydrolysis. Each data point represents the means ± S.D. of three independent measurements from two separate protein preparations.