FIGURE 8.

Immunoblot analysis of Congo red-induced T3SS secretion profiles for engineered Spa47 mutant Shigella strains. The levels of IpaB and IpaC actively secreted following Congo red induction were compared for Shigella strains expressing engineered ATPase inactive Spa47 mutants, as well as positive and negative controls expressing wild-type Spa47 and a spa47 null strain, respectively. A, actively secreted IpaB and IpaC were detected by Western blot and compared using densitometry analysis. B, the protein levels are reported relative to a S. flexneri strain expressing wild-type Spa47 (normalized to 100%) and are reported for each strain. The reported values represent the means ± S.D. from three independent analyses.