FIGURE 3.

LOXL2 is the main lysyl oxidase expressed in PFHR-9 matrix. A, the qPCR studies show the detection of LOXL2 as the only LOX isoform expressed in PFHR-9 cells. Error bars represent S.D. from three independent experiments. B, Western blotting detection of LOXL2 protein in a 4 m urea extract from PFHR-9 matrix. Detection of unprocessed (U) and processed (P) forms of LOXL2 from PFHR-9 matrix is similar to the profile observed for human recombinant LOXL2 (hrLOXL2) that was used as a control. C, mass spectrometry identification of mouse LOXL2 (mLOXL2) peptides extracted from PFHR-9 matrix (gray) and glomerular ECM (black).