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. 2016 Oct 25;291(50):26013–26023. doi: 10.1074/jbc.M116.756007

FIGURE 5.

FIGURE 5.

Binding of WT TrCel7A and its W38A variant to BC. Total bound, WT (■) and W38A (♦); active site bound WT (□) and W38A (♢); bound enzyme with free active site, WT (▵) and W38A (▴). The concentration of total bound enzyme ([TrCel7A]bound-tot) was found as a difference between the total enzyme concentration and the concentration of enzyme free in the solution. Concentration of active site bound enzyme ([TrCel7A]bound-OA) was found from the strength of inhibition of MUL hydrolysis by BC. Concentration of bound enzyme with the free active site ([Cel7A]bound-FA) was found as a difference between [TrCel7A]bound-tot and [TrCel7A]bound-OA. Error bars show S.D. and are from three independent experiments.