TABLE 1.

Kinetic parameters of TrCel7A and its variants measured with crystalline celluloses

Enzyme	Avicel			rBC
	koffa	Kmb	konc	kIRGd	Pappe
	10−3 s−1	g l−1	10−3 l g−1s−1	10−3 s−1
WT	0.7 ± 0.1	1.54 ± 0.23	0.46 ± 0.07	0.65 ± 0.03	70 ± 10
W38A	2.3 ± 0.1	5.95 ± 0.34	0.39 ± 0.02		32 ± 1
WTCD	1.7 ± 0.2	12.6 ± 1.82	0.14 ± 0.02	1.17 ± 0.25	58 ± 7

^a The k_off values measured using SEE (Fig. 2).

^b Data from Kari et al. (22).

^c k_on values are calculated according to k_on = k_off/K_m using listed k_off and K_m values.

^d k_IRG represents the off-rate constant measured from the generation of IRGs on rBC (Fig. 3B).

^e P_app is found as a slope of the linear regression line of the data in coordinates [RG]_tot versus [IRG] (Fig. 3C).