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The binding of BDD-fVIII to LRP1 is ionic strength-dependent. A Debye-Hückel plot of BDD-fVIII binding to LRP1 is shown. The KD value at each ionic strength (150, 250, 500, and 1,000 mm NaCl) was measured by equilibrium SPR measurements. The values represent the mean of three independent experiments. Error bars represent S.E. A slope of 1.8 ± 0.5 was determined by linear regression analysis (r2 = 0.7).
