FIGURE 3.

Binding of FL-fVIII and BDD-fVIII to LRP1 fits a bivalent binding model. A, bivalent binding model for the interaction of two distinct regions on fVIII with complementary sites on LRP1. B and C, increasing concentrations of FL-fVIII (2.5, 7.4, 22.2, 66.6, and 200 nm) (B) or BBD-fVIII (12.5, 25, 50, 100, and 200 nm) (C) were injected over the LRP1-coupled chip. The experimental data are shown in black, whereas fits to the bivalent binding model are shown in blue. Three independent experiments were performed, and representative experiments are shown. RU, resonance units.