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. 2016 Nov 1;291(50):26045–26055. doi: 10.1074/jbc.M116.758839

FIGURE 5.

FIGURE 5.

Effects of paraquat on proboscis extension in wild type and FK506-BP2 mutant flies. A, schematic depiction of the experimental design. B and C, graphs represent average values for maximum (B) and average velocity (C) on days 7 and 14 of wild type flies treated with vehicle (veh, gray diamonds), paraquat (black squares), and paraquat + S107 (red triangles) from 7 to 14 days of age (n = 23–32 events from 12–16 flies). Error bars indicate S.E. Significance was determined using ANOVA. *, p < 0.05; **, p < 0.01. D, immunoblot of 4-HNE-modified proteins from 14-day-old whole wild type fly extracts treated with vehicle, paraquat, and paraquat + S107 from 7 to 14 days of age. Tubulin is shown as a loading control. E and F, graphs represent average value for intensity of regions of interest (ROI) indicated in D normalized to tubulin. v, vehicle (gray bars); p, paraquat (black bars), p + s, paraquat + S107 (red bars) (n = 4). Error bars indicate S.D. Significance was determined using ANOVA. *, p < 0.05. G and H, graphs represent average values for maximum (G) and average velocity (H) on days 7 and 14 from FK506-BP2 flies treated with vehicle (gray diamonds), paraquat (black squares), and paraquat + S107 (red triangles) from 7 to 14 days of age (n = 25–32 events from 14–16 flies). Error bars indicate S.E. Significance was determined using ANOVA. *, p < 0.05; **, p < 0.01. I, immunoblot of 4-HNE-modified proteins from 14-day-old whole FK506-BP2 fly extracts treated with vehicle, paraquat, and paraquat + S107 from 7 to 14 days of age. Tubulin is shown as a loading control. J and K, graphs represent average value for intensity of regions of interest indicated in I normalized to tubulin. v, vehicle (gray bars); p, paraquat (black bars); p + s, paraquat + S107 (red bars) (n = 4). Error bars indicate S.D. Significance was determined using ANOVA. *, p < 0.05.