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. 2016 Oct 27;291(50):26083–26097. doi: 10.1074/jbc.M116.757138

FIGURE 1.

FIGURE 1.

Disruption of the β-arrestin1·STAM1 complex attenuates CXCR4-dependent chemotaxis. HeLa cells transiently transfected with empty vector (pCMV), FLAG-β-arrestin1(25–161), or FLAG-STAM1(296–380) were passaged onto μ-Slide chemotaxis chambers and analyzed by time lapse microscopy for 18 h in the absence (vehicle) or presence of a gradient of CXCL12 (50 nm at its source). A and B, aggregated trajectories of individual cells in the presence of vehicle (A) or CXCL12 (B) from a representative experiment. Trajectories in black and red represent cells that migrated toward or away from the chemoattractant gradient, respectively. C, the graph represents the mean forward migration index in the absence or presence of CXCL12 from tracking 150 cells from three (vehicle) or 200 cells from four (CXCL12) independent experiments. Data were analyzed by two-way ANOVA followed by Tukey's multiple comparison test. p values are provided. D, aggregated trajectories of individual cells in the presence of EGF (200 ng/ml at its source) from a representative experiment. E, the forward migration index is shown from tracking 100 cells from two independent experiments. The error bars represent the S.D. Data were analyzed by Student's t test and are not significant (ns).