FIGURE 4.

Depletion of FAK attenuates CXCR4-dependent chemotaxis. A and B, chemotaxis of HeLa cells transfected with control siRNA (siCtrl) or siRNA against FAK was analyzed as in Fig. 3. Aggregated trajectories of individual cells transfected with either control or FAK siRNA in the presence of vehicle (A) or CXCL12 gradient (B) are shown. C, the forward migration index was calculated from 200 vehicle (two experiments)- or CXCL12 (three experiments)-treated cells that were transfected with control siRNA and 300 vehicle (three experiments)- or 400 CXCL12 (four experiments)-treated cells that were transfected with FAK siRNA. The error bars represent the S.D. Data were analyzed by two-way ANOVA followed by Tukey's multiple comparison test. p values are provided.