FIGURE 9.

FAK exists in a CXCR4-promoted complex with β-arrestin1 and STAM1. HeLa cells transfected with β-arrestin1-FLAG and T7-STAM1 were treated with 10 nm CXCL12 for 5, 15, 30, or 60 min and vehicle for 60 min. For controls, HeLa cells transfected with pCMV (empty vector) were treated with vehicle or 10 nm CXCL12 for 30 min. Cleared lysates were immunoprecipitated (IP) with an anti-FLAG antibody. Immunoprecipitates and lysates were analyzed by immunoblotting for the indicated proteins. Representative immunoblots from three independent experiments are shown.