FIGURE 1.
Known anti-prion compounds are active in the DBCA. A, RML-infected ScN2a cells were treated for a total of 1 week with DMSO vehicle, or with the indicated concentrations of Congo Red (CR), tetrapyrrole (TMPyP), or pentosan polysulfate (PPS). Cells were lysed, and PK-treated lysates were analyzed by Western blotting for PrPSc. Molecular size markers are given in kDa. B, quantification of PrPSc levels, expressed relative to DMSO control (mean ± S.D., n = 3 cultures). C and D, dose-response curves of the activity of CR, TMPyP, and PPS in the DBCA. Compounds were assayed for their ability to restore cell viability to ΔCR PrP-expressing HEK cells exposed to G418. Cell viability was measured by MTT reduction. Error bars represent mean ± S.D., n = 3 cultures.