FIGURE 5.
JZ107 does not alter total or cell surface levels of PrPC. A, structure of JZ107, an active derivative. B, N2a.3 cells were treated for 1 week with the indicated concentrations of JZ107, after which cells were lysed and PrPC levels were evaluated by Western blotting. Actin was detected as a loading control. C, bars represent mean ± S.D. (n = 3 cultures) of PrPC levels, expressed relative to a DMSO control, and normalized for the content of actin. There was no statistically significant difference between any of the bars. D, unpermeabilized N2a.3 cells were stained for surface PrPC (red) after treatment for 1 week with DMSO vehicle, JZ107, or PPS. Nuclei were visualized by staining with Hoechst 33342 (blue). Scale bar = 10 μm. Images are representative of results from 3 independent experiments.