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. 2016 Oct 31;291(50):26177–26187. doi: 10.1074/jbc.M116.743906

FIGURE 1.

FIGURE 1.

The influence of PIs on latency cell models and primary CD4+ T cells from patients. A, the percentage of GFP-positive cells was measured by FCM after treatment with MG-132, BTZ, and CFZ on J-lat 10.6 for 48 h at the indicated concentrations. B, P24 in culture supernatant of ACH2 was detected by ELISA after treatment for 48 h with PIs at the indicated concentrations. C, CC50 of PIs in three cell types used in this study. D, the cell viability of ACH2, J-lat 10.6, and PBMCs were evaluated by CCK-8 after treatment for 48 h with PIs. E, primary CD4+ T cells isolated from three suppressive HIV+ patients were co-treated for 48 h with CFZ (20 nm) and three classical LRAs (SAHA, JQ1, and prostratin), and the transcription of HIV 5′-LTR was analyzed by qPCR. Data are reported as the mean ± S.E. from three independent experiments. F, J-lat 10.6 cells were co-treated for 48 h with CFZ (20 nm) and three classical LRAs (SAHA, JQ1, and prostratin), and the percentage of GFP-positive cells was measured by FCM. The p values were defined as: *, p < 0.05; **, p < 0.01; and ***, p < 0.001 versus control.