FIGURE 4.

Transcriptional elongation factor p-TEFb participates in the reactivation of latent HIV under proteasome inhibition. A, the accumulation of CDK9 in the nucleus induced by PIs in 2 h was detected by Western blotting. B, PIs were co-treated with p-TEFb inhibitor (DRB) at the indicated concentrations for 48 h. Then the percentage of positive GFP cells was measured by FCM and (C) the corresponding total protein level was measured by Western blotting analysis. D, HSF1 antibody pulled down the complex of p-TEFb and HPS90 treated with CFZ at 48 h. *, p < 0.05 and ***, p < 0.001 versus control.