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. 2016 Oct 31;291(50):26177–26187. doi: 10.1074/jbc.M116.743906

FIGURE 5.

FIGURE 5.

HSP90 positively regulates the latent HIV reactivation induced by PIs. A, J-lat 10.6 cells were treated with CFZ (40 nm) for 12 h and the mRNA expression of HSPs and stress kinases were detected by qPCR. B, total protein indicated in the figure was measured after co-treatment with CFZ and 17-DMAG for 48 h on J-lat 10.6 cells. C, the percentage of GFP-positive cells was measured by FCM after co-treatment for 48 h with PIs and HSP90 inhibitors. D, the percentage of GFP-positive cells was measured by FCM after treatment with NVP-AUY922 and 17-DMAG on J-lat 10.6 for 48 h. E, the expression of HSP90 was detected by Western blotting analysis after treatment for 48 h with NVP-AUY922 and 17-DMAG on J-lat 10.6. F, the knockdown of HSP90 was verified by detecting the expression of HSP90 after electroporating siRNA into J-lat 10.6 cells for 48 h. G, the percentage of GFP-positive cells was measured by FCM after treating with PIs on HSP90-knockdown cells for 48 h. *, p < 0.05; **, p < 0.01; and ***, p < 0.001 versus control.