Subcellular localization of HDAg-L, HDAg-S, TAP, and Aly in Huh7 cells. Huh7 cells were left untreated or were transfected with plasmid pECE-d-BE or pECE-d-SM containing cDNA encoding, respectively, HDAg-L or HDAg-S, then the subcellular localization of HDAg-L or HDAg-S and either TAP (A) or Aly (B) was examined by immunofluorescence staining with antibodies against HDAg, TAP, or Aly and confocal microscopy. NT, non-transfected. The bars on the images represent 20 μm.