FIGURE 5.

The HDAg-L NLS deletant mutant does not bind to TAP. A, Huh7 cells were transfected with plasmid pECEL-d35/88, then, at 2 days posttransfection, the subcellular localization of HDAg-L-d35/88 and TAP was examined by immunofluorescence staining with antibodies against HDAg and TAP using confocal microscopy. The bars on the images represent 20 μm. B, Huh7 cells were transfected with plasmid pECE-d-BE, coding for full-length HDAg-L, or pECEL-d35/88 lacking residues 35–88, then, at 2 days posttransfection, were harvested and the cell lysate was subjected to immunoprecipitation (IP) with anti-TAP antibodies, followed by Western blotting (WB) analysis with antibodies against TAP or HDAgs. The positions of the molecular weight markers are shown on the left. NT, non-transfected.