Figure 4:

A, Photograph shows microintraesophageal agent–delivery and RF-heating system. B, US image shows guided positioning of microagent delivery system (arrow) and then intraesophageal inoculation of the target esophageal region with luciferase-labeled human esophageal carcinoma cells through the tip-curved needle (arrowhead). C, US image shows subsequent injection of cell pellet, which appears as an inhomogeneous hyperechoic signal intensity (arrow). D, Optical image shows bioluminescence signal from the generated esophageal tumor. E, Gross specimen displays tumor (arrow) adhering to esophageal wall (arrowheads). F, Photomicrograph (hematoxylin and eosin–stain; magnification, ×4) further confirms esophageal carcinoma (EC) with tumor invasion (arrows). RFH = RF heat.