TABLE 1.
Summary of kinetic parameters
Data are means ± S.E. n = 2–4 independent experiments, each with nine independent measurements. Vmax differences between ZnT8-R and ZnT8-W are as follows: ns, p > 0.1, not statistically significant; **, p < 0.02, statistically significant; and ***, p < 0.01 very statistically significant.
| Lipid compositions | ZnT8 variant ZnT8-R |
ZnT8 variant ZnT8-W |
||
|---|---|---|---|---|
| Vmax (0.01s−1) | Km (μm) | Vmax (0.01s−1) | Km (μm) | |
| PC/PE/PG | 12.68 ± 0.312*** | 141.31 ± 30.23 | 8.07 ± 0.60 | 182.93 ± 42.71 |
| 2:1:1 | ||||
| PC/PE/PG | 7.69 ± 0.39*** | 259.74 ± 36.31 | 4.73 ± 0.35 | 219.34 ± 46.82 |
| 1:1:1 | ||||
| PC/PE/PG | 6.39 ± 0.54*** | 430.14 ± 86.42 | 4.36 ± 0.27 | 414.43 ± 62.43 |
| 1:2:1 | ||||
| DOPC/DOPE/DOPS | 10.83 ± 0.43*** | 165.51 ± 21.13 | 7.70 ± 0.40 | 152.92 ± 26.04 |
| 2:1:1 | ||||
| DOPC/DOPE/soyPI | 8.35 ± 0.33ns | 131.01 ± 17.72 | 7.67 ± 0.25 | 125.23 ± 14.33 |
| 2:1:1 | ||||
| DOPC/DOPE | 3.26 ± 0.28*** | 71.81 ± 24.43 | 1.87 ± 0.18 | 181.62 ± 54.72 |
| 2:1 | ||||
| PC/PE/PI/PS | 6.22 ± 0.38*** | 103.12 ± 22.23 | 4.07 ± 0.19 | 162.21 ± 24.41 |
| 45:20:23:12 | ||||
| PC/PE/PI/PS + SM | 5.84 ± 0.37*** | 125.61 ± 25.21 | 3.73 ± 0.24 | 104.82 ± 24.33 |
| 45:20:23:12 + 10% | ||||
| PC/PE/PI/PS + chol | 6.41 ± 0.41ns | 71.42 ± 17.63 | 5.60 ± 0.37 | 56.42 ± 14.81 |
| 45:20:23:12 + 5% | ||||
| PC/PE/PI/PS + chol + SM | 4.12 ± 0.23** | 116.93 ± 22.41 | 3.36 ± 0.18 | 103.31 ± 19.62 |
| 45:20:23:12 + 5% + 10% | ||||
| PC/PE/PS/PI/LPC + SM + Chol | 3.43 ± 0.14*** | 154.71 ± 21.33 | 2.88 ± 0.10 | 318.53 ± 30.13 |
| 2:1:1:1:1 + 10% + 5% | ||||
| PC/PE/PS/LPC | 2.06 ± 0.33*** | 465.14 ± 180.11 | 1.32 ± 0.08 | 101.61 ± 22.63 |
| 1:1:1:1 | ||||
| PC/PE/LPC/PS + chol | 3.14 ± 0.21*** | 93.42 ± 36.94 | 2.0 ± 0.15 | 166.94 ± 41.12 |
| 2:1:1:1 + 5% | ||||
| PC/PE/PG + chol | 2.57 ± 0.32ns | 134.04 ± 56.12 | 2.59 ± 0.24 | 160.32 ± 47.33 |
| 2:1:1 + 5% | ||||
| PC/PE/PS + chol | 1.60 ± 0.13*** | 360.81 ± 74.43 | 1.08 ± 0.17 | 433.54 ± 162.84 |
| 2:1:1 + 5% | ||||