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. 2016 Nov 8;291(53):26958–26969. doi: 10.1074/jbc.M116.735365

FIGURE 9.

FIGURE 9.

Intravital microscopy imaging of mouse brain demonstrates the protective effect of calpain inhibitor III against TBI-induced BBB hyperpermeability. Mice subjected to TBI demonstrated a significant increase in BBB permeability at 60 min as evidenced by enhanced FITC-dextran fluorescence compared with the sham injury group (A and B, n = 5 each group, p < 0.05). Calpain inhibitor III (10 μg/g body weight of the animal; n = 4) treatment 15 min following TBI attenuated TBI-induced BBB hyperpermeability as evidenced by the extravasation of FITC-dextran into the extravascular space compared with TBI + vehicle group (A and B, p < 0.05). Data are expressed as a change in relative fluorescence to the initial image (0 time point) in each group, and the sham injury group is used as the baseline for all comparisons. *a indicates significant increase compared with the sham injury group; *b indicates significant decrease compared with the vehicle + TBI group.