TABLE 1.
Antibodies and their conditions used for immunostaining
NA, not applicable.
| Immunostaining | |||||
|---|---|---|---|---|---|
| Primary antibodies |
Secondary antibodies |
||||
| Name | Supplier, catalog no. | Working dilution/species | Name | Supplier, catalog no. | Working dilution |
| IBA-1 | Wako, 019-19741 | 1:200/rabbit | Anti-rabbit Alexa Fluor 488 | Invitrogen, A11008 | 1:5000 |
| GFAP | Abcam, 7260 | 1:250/rabbit | Anti-rabbit Alexa Fluor 488 | Invitrogen, A11008 | 1:5000 |
| NG2 | Millipore, AB5320 | 1:100/rabbit | Anti-rabbit Alexa Fluor 488 | Invitrogen, A11008 | 1:5000 |
| NeuN | Abcam, 104225 | 1:500/rabbit | Anti-rabbit Alexa Fluor 488 | Invitrogen, A11008 | 1:5000 |
| PDGFRβ | Abcam, 32570 | 1:100/rabbit | Anti-rabbit Alexa Fluor 488 | Invitrogen, A11008 | 1:5000 |
| CD31 | Abcam, 7388-50 | 1:100/rat | Anti-rat Alexa Fluor 488 | Invitrogen, A21208 | 1:5000 |
| TTR | Thermo Fisher, PA5-27220 | 1:100/rabbit | Anti-rabbit Alexa Fluor 488 | Invitrogen, A11008 | 1:5000 |
| Fluorescein-labeled lectin | Vector Laboratories, FL-1171 | 1:200 | NA | NA | NA |
| PA | Gift from Prof. E. W. Weiler | 1:50/mouse | Anti-mouse rhodamine | Invitrogen, A21208 | 1:5000 |
| MAP2 | Abcam, 32454 | 1:250/rabbit | Anti-rabbit Alexa Fluor 488 | Invitrogen, A11008 | 1:5000 |