FIGURE 5.

BITC rapidly down-regulates c-Myc. A, model of the proposed mechanism of action of BITC-induced ROS on down-regulation of STAT3 and Sp transcription factors is shown. B, L3.6pL, MiaPaCa2, and Panc1 cells were pretreated with 5 mm GSH for 3 h and then treated with 10 μm BITC for 4 h, and c-Myc protein expression was analyzed by Western blottings. The Western blottings in Figs. 4B and 5B were from the same experiment. C, L3.6pL, MiaPaCa2, and Panc1 cells were treated with 10 μm BITC alone for 3, 6, 9, 12, and 24 h, and whole cell lysates were analyzed and quantitated for c-Myc protein expression by Western blotting. D, Panc1 cells were treated with 10 μm BITC for 3 h, and ChIP assays were performed as in Fig. 4D on the Myc promoter.