FIGURE 1.

Establishment of in vitro OSCN-system. A, chemical reactions of in vitro H₂O₂ and OSCN⁻ production. B and C, medium containing 11 mm β-d-glucose, 3 mm HSCN, and GOX (closed square, 1 milliunit/ml; open triangle, 2 milliunits/ml; closed triangle, 4 milliunits/ml; open circle, 8 milliunits/ml, and closed circle, 16 milliunits/ml) was incubated without (B) or with (C) 10 μg/ml LPO for the indicated times. Produced H₂O₂ (B) or OSCN⁻ (C) is depicted. All experiments were performed at least more than two times.